Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: CD14 directs adventitial macrophage precursor recruitment: role in early abdominal aortic aneurysm formation.

Authors: Blomkalns, Andra L; Gavrila, Daniel; Thomas, Manesh; Neltner, Bonnie S; Blanco, Victor M; Benjamin, Stephanie B; McCormick, Michael L; Stoll, Lynn L; Denning, Gerene M; Collins, Sean P; Qin, Zhenyu; Daugherty, Alan; Cassis, Lisa A; Thompson, Robert W; Weiss, Robert M; Lindower, Paul D; Pinney, Susan M; Chatterjee, Tapan; Weintraub, Neal L

Published In J Am Heart Assoc, (2013 Mar 08)

Abstract: Recruitment of macrophage precursors to the adventitia plays a key role in the pathogenesis of abdominal aortic aneurysms (AAAs), but molecular mechanisms remain undefined. The innate immune signaling molecule CD14 was reported to be upregulated in adventitial macrophages in a murine model of AAA and in monocytes cocultured with aortic adventitial fibroblasts (AoAf) in vitro, concurrent with increased interleukin-6 (IL-6) expression. We hypothesized that CD14 plays a crucial role in adventitial macrophage precursor recruitment early during AAA formation.CD14(-/-) mice were resistant to AAA formation induced by 2 different AAA induction models: aortic elastase infusion and systemic angiotensin II (AngII) infusion. CD14 gene deletion led to reduced aortic macrophage infiltration and diminished elastin degradation. Adventitial monocyte binding to AngII-infused aorta in vitro was dependent on CD14, and incubation of human acute monocytic leukemia cell line-1 (THP-1) monocytes with IL-6 or conditioned medium from perivascular adipose tissue (PVAT) upregulated CD14 expression. Conditioned medium from AoAf and PVAT induced CD14-dependent monocyte chemotaxis, which was potentiated by IL-6. CD14 expression in aorta and plasma CD14 levels were increased in AAA patients compared with controls.These findings link CD14 innate immune signaling via a novel IL-6 amplification loop to adventitial macrophage precursor recruitment in the pathogenesis of AAA.

PubMed ID: 23537804 Exiting the NIEHS site

MeSH Terms: Adventitia/immunology; Animals; Aortic Aneurysm, Abdominal/immunology*; Cell Line, Tumor; Cell Migration Assays, Macrophage; Cell Movement/immunology*; Cells, Cultured; Disease Models, Animal; Humans; Immunity, Innate; Interleukin-6/immunology*; Lipopolysaccharide Receptors/immunology*; Macrophages, Peritoneal; Macrophages/immunology*; Mice; Mice, Transgenic; Monocyte-Macrophage Precursor Cells/immunology*; Signal Transduction/immunology

to Top