Title: Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega-3 fatty acids.

Authors: Lundström, Susanna L; Yang, Jun; Brannan, John D; Haeggström, Jesper Z; Hammock, Bruce D; Nair, Parameswaran; O'Byrne, Paul; Dahlén, Sven-Erik; Wheelock, Craig E

Published In Mol Nutr Food Res, (2013 Aug)

Abstract: In contrast to well-characterized PUFA levels in serum, little is known regarding their downstream metabolic products. However, many of these compounds are lipid mediators with prominent roles during pro- and antiinflammatory processes.In this double blind crossover study on asthmatics, shifts in serum levels of ω-3 and ω-6 PUFA-derived oxidized fatty acids (e.g. eicosanoids, oxylipins) were quantified following dietary fish oil supplementation. Serum was obtained from subjects following fasting at three occasions; (i) prior to supplementation, (ii) following a 3-week supplement intake of either placebo or fish oil, and (iii) following a 3-week washout period with a subsequent 3-week period of either fish oil or placebo supplement. A total of 87 oxylipins representing cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP) metabolic pathways were screened via LC-MS/MS. The primary alterations observed were in CYP- and 15-LOX-derived EPA- and CYP-derived DHA oxylipins.The results indicate that intake of an ω-3 rich diet alters not only the PUFA ratio, but also the ratio of downstream oxylipins. These data further support that dietary manipulation with ω-3 PUFAs affects not only PUFA levels, but importantly also the downstream metabolic profile.

PubMed ID: 23824870

MeSH Terms: Adult; Asthma/diet therapy*; Dietary Supplements; Docosahexaenoic Acids/blood; Eicosapentaenoic Acid/blood; Fatty Acids, Omega-3/blood; Fatty Acids, Omega-3/therapeutic use*; Female; Humans; Male; Middle Aged; Multivariate Analysis; Oxylipins/blood*; Phospholipids/blood; Phospholipids/chemistry; Young Adult