Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Elemental bioimaging of manganese uptake in C. elegans.

Authors: Brinkhaus, Sigrid Grosse; Bornhorst, Julia; Chakraborty, Sudipta; Wehe, Christoph A; Niehaus, Rebecca; Reifschneider, Olga; Aschner, Michael; Karst, Uwe

Published In Metallomics, (2014 Mar)

Abstract: A new method for elemental bioimaging with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was developed and applied to study the uptake of manganese (Mn) in Caenorhabditis elegans (C. elegans). C. elegans is a well-established model organism in neuroscience, genetics and genomics, which has been extensively studied to decipher mechanisms of heavy metal induced toxicity. Knowledge about the distribution of manganese (Mn) and other metals in this organism will be helpful in elucidating pathways and mechanisms of transport, distribution and excretion. The LA-ICP-MS method requires limited sample preparation and can be used rapidly and easily to visualize the Mn distribution in C. elegans. Due to thorough optimization of the analytical parameters, intense Mn signals in C. elegans wild-type (WT) and mutants were obtained at a spatial resolution as small as 4 μm, thus proving the suitability of LA-ICP-MS to study the uptake of metals in C. elegans.

PubMed ID: 24481269 Exiting the NIEHS site

MeSH Terms: Animals; Biological Transport; Caenorhabditis elegans/chemistry; Caenorhabditis elegans/metabolism*; Laser Therapy; Manganese/analysis; Manganese/metabolism*; Mass Spectrometry

Back
to Top