Title: Vitamin E, γ-tocopherol, diminishes ex vivo basophil response to dust mite allergen.

Authors: Mills, K; Lay, J; Wu, W; Robinette, C; Kesic, M J; Dreskin, S C; Peden, D B; Hernandez, M

Published In Allergy, (2014 Apr)

Abstract: Epidemiologic studies suggest that dietary vitamin E is a candidate intervention for atopic disease. We used in vitro and ex vivo exposures to test the hypothesis that the most common dietary isoform of vitamin E, γ-tocopherol (γT), could suppress FcεRI-mediated basophil activation. Rat basophilic leukemia (RBL)-SX38 cells that express human FcεRI were treated with or without γT, followed by stimulation with α-IgE. In the ex vivo study, 20 Der f 1-allergic volunteers consumed a γT-enriched supplement for 7 days. Their basophils were challenged ex vivo with α-IgE and graded doses of Der f 1 before and after the supplementation period. γt treatment of RBL-SX38 cells significantly reduced basophil degranulation and de novo TH2 cytokine production. Daily consumption of a γT-rich supplement by dust mite-allergic volunteers reduced basophil activation after ex vivo dust mite challenge. Vitamin E supplements rich in γT may be useful adjuncts in decreasing atopic disease.

PubMed ID: 24697338

MeSH Terms: Antigens, Dermatophagoides/immunology*; Basophils/drug effects*; Basophils/immunology*; Cell Degranulation/drug effects; Cell Degranulation/immunology; Cell Line; Cytokines/biosynthesis; Humans; Immunoglobulin E/immunology; Leukotriene D4/metabolism; Vitamin E/pharmacology*; gamma-Tocopherol/pharmacology*