Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Quantitative detection of 8-Oxo-7,8-dihydro-2'-deoxyguanosine using chemical tagging and qPCR.

Authors: Bajacan, John Ernest Vallarta; Hong, In Seok; Penning, Trevor M; Penning, Trevor W; Greenberg, Marc M

Published In Chem Res Toxicol, (2014 Jul 21)

Abstract: 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is a commonly formed DNA lesion that is useful as a biomarker for oxidative stress. Although methods for selective quantification of 8-oxodGuo exist, there is room for additional methods that are sensitive and utilize instrumentation that is widely available. We previously took advantage of the reported reactivity of 8-oxodGuo to develop a method for detecting the lesion by selectively covalently tagging it with a molecule equipped with a biotin label that can be used subsequently with a reporting method ( Xue , L. and Greenberg , M. M. ( 2007 ) J. Am. Chem. Soc. 129 , 7010 ). We now report a method that can detect as little as 14 amol of 8-oxodGuo by tagging DNA with a reagent containing a disulfide that reduces background due to nonspecific binding. The reagent also contains biotin that enables capturing target DNA on streptavidin-coated magnetic beads. The captured DNA is quantified using quantitative PCR. The method is validated by comparing the amount of 8-oxodGuo detected as a function of Fe(2+)/H2O2/ascorbate-dose to that reported previously using mass spectrometry.

PubMed ID: 24932862 Exiting the NIEHS site

MeSH Terms: Ascorbic Acid/chemistry; Biotin/chemistry; Deoxyguanosine/analogs & derivatives*; Deoxyguanosine/analysis; Deoxyguanosine/chemistry; Disulfides/chemistry; Ferrous Compounds/chemistry; Hydrogen Peroxide/chemistry; Plasmids; Polymerase Chain Reaction; Streptavidin/chemistry; Tumor Suppressor Protein p53/genetics

to Top