Title: Preparation of rodent testis co-cultures.
Authors: Wegner, Susanna; Hong, Sungwoo; Yu, Xiaozhong; Faustman, Elaine M
Published In Curr Protoc Toxicol, (2013 Feb)
Abstract: Male reproductive development is a complex process that is sensitive to disruption by a range of toxicants. There is a great need for in vitro models that can evaluate potential male reproductive toxicants. The current unit presents a protocol for preparation of a three-dimensional in vitro model of male reproductive development that reduces the number of animals required for evaluation of toxicants. A Matrigel overlay provides a three-dimensional extracellular matrix that improves cell attachment, viability, and communication, and makes the model more reflective of in vivo environments.
PubMed ID: 23408194
MeSH Terms: Animal Testing Alternatives/methods*; Animals; Cell Culture Techniques/methods*; Cell Survival/drug effects; Cells, Cultured; Coculture Techniques/methods; Leydig Cells/cytology; Leydig Cells/drug effects; Male; Rats; Sertoli Cells/cytology; Sertoli Cells/drug effects; Spermatozoa/cytology; Spermatozoa/drug effects; Testis*/cytology; Testis*/drug effects; Toxicity Tests/methods*