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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products.

Authors: Hua, Xiude; Yin, Wei; Shi, Haiyan; Li, Ming; Wang, Yanru; Wang, Hong; Ye, Yonghao; Kim, Hee Joo; Gee, Shirley J; Wang, Minghua; Liu, Fengquan; Hammock, Bruce D

Published In Anal Chem, (2014 Aug 19)

Abstract: Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.

PubMed ID: 25135320 Exiting the NIEHS site

MeSH Terms: Amino Acid Sequence; Antibodies, Monoclonal/chemistry; Bacteriophages/chemistry*; Base Sequence; Brassica/chemistry; DNA/chemistry; Food Analysis/economics; Food Analysis/methods*; Immunoassay/economics; Immunoassay/methods*; Limit of Detection; Malus/chemistry; Molecular Sequence Data; Nucleic Acid Amplification Techniques/economics; Nucleic Acid Amplification Techniques/methods; Organophosphorus Compounds/analysis*; Peptides/chemistry*; Pesticides/analysis*

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