Title: Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.
Authors: Wagner, Jeffrey C; Platt, Randall J; Goldfless, Stephen J; Zhang, Feng; Niles, Jacquin C
Published In Nat Methods, (2014 Sep)
Abstract: Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥ 50-100%) gene disruption frequencies within the usual time frame for generating transgenic parasites.
PubMed ID: 25108687
MeSH Terms: Animals; Animals, Genetically Modified/genetics*; Base Sequence; CRISPR-Cas Systems/genetics*; Genetic Engineering/methods*; Genome/genetics*; Molecular Sequence Data; Plasmodium falciparum/genetics*; RNA Editing/genetics*