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Title: Effects of soluble epoxide hydrolase deficiency on acute pancreatitis in mice.

Authors: Bettaieb, Ahmed; Chahed, Samah; Tabet, George; Yang, Jun; Morisseau, Christophe; Griffey, Stephen; Hammock, Bruce D; Haj, Fawaz G

Published In PLoS One, (2014)

Abstract: Acute pancreatitis (AP) is a frequent gastrointestinal disorder that causes significant morbidity, and its incidence has been progressively increasing. AP starts as a local inflammation in the pancreas that often leads to systemic inflammatory response and complications. Soluble epoxide hydrolase (sEH) is a cytosolic enzyme whose inhibition in murine models has beneficial effects in inflammatory diseases, but its significance in AP remains unexplored.To investigate whether sEH may have a causal role in AP we utilized Ephx2 knockout (KO) mice to determine the effects of sEH deficiency on cerulein- and arginine-induced AP. sEH expression increased at the protein and messenger RNA levels, as well as enzymatic activity in the early phase of cerulein- and arginine-induced AP in mice. In addition, amylase and lipase levels were lower in cerulein-treated Ephx2 KO mice compared with controls. Moreover, pancreatic mRNA and serum concentrations of the inflammatory cytokines IL-1B and IL-6 were lower in cerulein-treated Ephx2 KO mice compared with controls. Further, Ephx2 KO mice exhibited decreased cerulein- and arginine-induced NF-κB inflammatory response, MAPKs activation and decreased cell death. Conclusions -These findings demonstrate a novel role for sEH in the progression of cerulein- and arginine-induced AP.

PubMed ID: 25402489 Exiting the NIEHS site

MeSH Terms: Acute Disease; Animals; Cell Death/genetics; Ceruletide/adverse effects; Disease Models, Animal; Epoxide Hydrolases/deficiency*; Epoxide Hydrolases/genetics; Epoxide Hydrolases/metabolism; Gene Expression; MAP Kinase Signaling System; Male; Mice; Mice, Knockout; NF-kappa B/metabolism; Pancreatitis/chemically induced; Pancreatitis/enzymology; Pancreatitis/genetics*; Pancreatitis/pathology

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