Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Caspase-3-Dependent Cleavage of the Glutamate-L-Cysteine Ligase Catalytic Subunit during Apoptotic Cell Death.

Authors: Franklin, Christopher C; Krejsa, Cecile M; Pierce, Robert H; White, Collin C; Fausto, Nelson; Kavanagh, Terrance J

Published In Am J Pathol, (2002 May)

Abstract: Apoptotic cell death is usually accompanied by activation of a family of cysteine proteases termed caspases. Caspases mediate the selective proteolysis of multiple cellular targets often resulting in the disruption of survival pathways. Intracellular levels of the antioxidant glutathione (GSH) are an important determinant of cellular susceptibility to apoptosis. The rate-limiting step in GSH biosynthesis is mediated by glutamate-L-cysteine ligase (GCL), a heterodimeric enzyme consisting of a catalytic (GCLC) and a modifier (GCLM) subunit. In this report we demonstrate that GCLC is a direct target for caspase-mediated cleavage in multiple models of apoptotic cell death. Mutational analysis revealed that caspase-mediated cleavage of GCLC occurs at Asp(499) within the sequence AVVD(499)G. GCLC cleavage occurs upstream of Cys(553), which is thought to be important for association with GCLM. GCLC cleavage is accompanied by a rapid loss of intracellular GSH due to caspase-mediated extrusion of GSH from the cell. However, while GCLC cleavage is dependent on caspase-3, GSH extrusion occurs by a caspase-3-independent mechanism. Our identification of GCLC as a target for caspase-3-dependent cleavage during apoptotic cell death suggests that this post-translational modification may represent a novel mechanism for regulating GSH biosynthesis during apoptosis.

PubMed ID: 12000740 Exiting the NIEHS site

MeSH Terms: Animals; Apoptosis/drug effects; Apoptosis/physiology*; Base Sequence; Binding Sites/genetics; Caspase 3; Caspases/metabolism*; Catalytic Domain; Dose-Response Relationship, Drug; Enzyme Activation; Glutamate-Cysteine Ligase/genetics; Glutamate-Cysteine Ligase/metabolism*; Glutathione/metabolism; HeLa Cells; Humans; Jurkat Cells; Mice; Receptors, Cell Surface/physiology; Time Factors; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha/pharmacology

Back
to Top