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Title: SP-D-dependent regulation of NO metabolism in lipopolysaccharide-stimulated peritoneal macrophages.

Authors: Atochina-Vasserman, E N; Abramova, E V; Tomer, Y; Scott, P; Nazarov, V A; Kruglov, S V; Beers, M F; Gow, A J; Malyshev, I Yu

Published In Bull Exp Biol Med, (2009 Apr)

Abstract: This work was designed to study the role of surfactant protein D in the regulation of NO synthesis by "non-alveolar" microphages. We evaluated whether the effects of surfactant protein D depend on the phenotype of macrophages. In the absence of surfactant protein D, the LPS-induced iNOS response was shown to decrease in macrophages of native and proinflammatory phenotypes by 30%, and in macrophages of the antiinflammatory phenotype (by 63%). Under the influence of lipopolysaccharide in high doses (500 ng/ml), NO(2)*- production by mouse macrophages without surfactant protein D was reduced in native cells (by 25%), but increased in proinflammatory (by 40%) and antiinflammatory phenotypes (by 12% compared to mouse macrophages with surfactant protein D). Our results suggest that surfactant protein D is involved in the immune response in the whole organism, but not only in the lungs. The effect of surfactant protein D depends on the phenotype of macrophages.

PubMed ID: 19704937 Exiting the NIEHS site

MeSH Terms: Animals; Cells, Cultured; Cytokines/metabolism; Lipopolysaccharides/toxicity*; Macrophages/cytology; Macrophages/drug effects*; Macrophages/physiology*; Mice; Mice, Inbred C57BL; Mice, Knockout; Nitric Oxide Synthase Type II/metabolism; Nitric Oxide/metabolism*; Nitrites/metabolism; Peritoneal Cavity/cytology; Peritoneal Cavity/physiopathology*; Pulmonary Surfactant-Associated Protein D/genetics; Pulmonary Surfactant-Associated Protein D/metabolism*; Reverse Transcriptase Polymerase Chain Reaction; Time Factors

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