Title: Solar-simulated ultraviolet radiation induces histone 3 methylation changes in the gene promoters of matrix metalloproteinases 1 and 3 in primary human dermal fibroblasts.
Authors: Gesumaria, Lisa; Matsui, Mary S; Kluz, Thomas; Costa, Max
Published In Exp Dermatol, (2015 May)
Abstract: Molecular signalling pathways delineating the induction of matrix metalloproteinases (MMPs) by ultraviolet radiation (UVR) are currently well-defined; however, the effects of UVR on epigenetic mechanisms of MMP induction are not as well understood. In this study, we examined solar-simulated UVR (ssUVR)-induced gene expression changes and alterations to histone methylation in the promoters of MMP1 and MMP3 in primary human dermal fibroblasts (HDF). Gene expression changes, including the increased expression of MMP1 and MMP3, were observed using Affymetrix GeneChip arrays and confirmed by qRT-PCR. Using ChIP-PCR, we showed for the first time that in HDF irradiated with 12 J/cm(2) ssUVR, the H3K4me3 transcriptional activating mark increased and the H3K9me2 transcriptional silencing mark decreased in abundance in promoters, correlating with the observed elevation of MMP1 and MMP3 mRNA levels following ssUVR exposure. Changes in mRNA levels due to a single exposure were transient and decreased 5 days after exposure.
PubMed ID: 25707437
MeSH Terms: Cells, Cultured; Epigenesis, Genetic/radiation effects; Fibroblasts/metabolism; Fibroblasts/radiation effects; Histones/chemistry; Histones/metabolism*; Histones/radiation effects*; Humans; Matrix Metalloproteinase 1/genetics*; Matrix Metalloproteinase 3/genetics*; Methylation/radiation effects; Promoter Regions, Genetic/radiation effects; RNA, Messenger/genetics; RNA, Messenger/metabolism; Skin Aging/genetics; Skin Aging/radiation effects; Skin/metabolism*; Skin/radiation effects*; Ultraviolet Rays/adverse effects*