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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Characterization of a novel angular dioxygenase from fluorene-degrading Sphingomonas sp. strain LB126.

Authors: Schuler, Luc; Ní Chadhain, Sinéad M; Jouanneau, Yves; Meyer, Christine; Zylstra, Gerben J; Hols, Pascal; Agathos, Spiros N

Published In Appl Environ Microbiol, (2008 Feb)

Abstract: In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The alpha and beta subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran degrader Terrabacter sp. strain DBF63, were identified. When overexpressed in Escherichia coli, FlnA1-FlnA2 was responsible for the angular oxidation of fluorene, 9-hydroxyfluorene, 9-fluorenone, dibenzofuran, and dibenzo-p-dioxin. Moreover, FlnA1-FlnA2 was able to oxidize polycyclic aromatic hydrocarbons and heteroaromatics, some of which were not oxidized by the dioxygenase from Terrabacter sp. strain DBF63. The quantification of resulting oxidation products showed that fluorene and phenanthrene were the preferred substrates of FlnA1-FlnA2.

PubMed ID: 18156320 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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