Title: Selective modulation of the functions of a conserved DNA motor by a histone fold complex.

Authors: Xue, Xiaoyu; Choi, Koyi; Bonner, Jacob N; Szakal, Barnabas; Chen, Yu-Hung; Papusha, Alma; Saro, Dorina; Niu, Hengyao; Ira, Grzegorz; Branzei, Dana; Sung, Patrick; Zhao, Xiaolan

Published In Genes Dev, (2015 05 15)

Abstract: Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes. Here, we show that the conserved histone fold MHF complex promotes Mph1-mediated repair of damaged replication forks but does not influence the outcome of DNA double-strand break repair. Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replication-associated repair through binding to Mph1 but not DNA. Thus, scMHF is a function-specific enhancer of Mph1 that enables flexible response to different genome repair situations.

PubMed ID: 25956905

MeSH Terms: DEAD-box RNA Helicases/metabolism; DNA Repair; DNA-Binding Proteins/metabolism*; DNA/genetics; DNA/metabolism*; Genome, Fungal/genetics; Histones/metabolism*; Mutation; Protein Binding; Protein Folding; Protein Structure, Tertiary; Recombination, Genetic; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism*; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology*