Skip Navigation

Publication Detail

Title: High-speed MALDI MS/MS imaging mass spectrometry using continuous raster sampling.

Authors: Prentice, Boone M; Chumbley, Chad W; Caprioli, Richard M

Published In J Mass Spectrom, (2015 Apr)

Abstract: A matrix-assisted laser desorption/ionization time of flight/time of flight tandem mass spectrometer (MALDI TOF/TOF) has been used for high-speed precursor/fragment ion transition image acquisition. High-throughput analysis is facilitated by an Nd:YLF solid state laser capable of pulse repetition rates up to 5 kHz, a high digitizer acquisition rate (up to 50 pixels/s), and continuous laser raster sampling. MS/MS experiments are enabled through the use of a precision timed ion selector, second source acceleration, and a dedicated collision cell. Continuous raster sampling is shown here to facilitate rapid MS/MS ion image acquisition from thin tissue sections for the drug rifampicin and for a common kidney lipid, SM4s(d18:1/24:1). The ability to confirm the structural identity of an analyte as part of the MS/MS imaging experiment is an essential part of the analysis. Additionally, the increase in sensitivity and specificity afforded by an MS/MS approach is highly advantageous, especially when interrogating complex chemical environments such as those in biological tissues. Herein, we report continuous laser raster sampling TOF/TOF imaging methodologies which demonstrate 8 to 14-fold increases in throughput compared with existing MS/MS instrumentation, an important advantage when imaging large areas on tissues.

PubMed ID: 26149115 Exiting the NIEHS site

MeSH Terms: Animals; Brain Chemistry; Histocytochemistry; Kidney/chemistry; Lipids/analysis; Lipids/chemistry; Liver/chemistry; Mice; Molecular Imaging/methods*; Pharmaceutical Preparations/analysis; Pharmaceutical Preparations/chemistry; Rats; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*; Tandem Mass Spectrometry/methods*

Back
to Top