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Publication Detail

Title: Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1.

Authors: Petukhova, G; Sung, P; Klein, H

Published In Genes Dev, (2000 Sep 01)

Abstract: The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.

PubMed ID: 10970884 Exiting the NIEHS site

MeSH Terms: Adenosine Triphosphatases/metabolism; DNA Helicases; DNA Repair Enzymes; DNA Topoisomerases; DNA Topoisomerases, Type I/metabolism; DNA, Superhelical/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism*; DNA/metabolism; Electrophoresis, Agar Gel; Electrophoresis, Gel, Two-Dimensional; Escherichia coli/metabolism; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism*; Gene Expression Regulation, Fungal; Immunoblotting; Plasmids/metabolism; Protein Binding; Rad51 Recombinase; Rec A Recombinases/metabolism; Recombination, Genetic; Saccharomyces cerevisiae Proteins*; Saccharomyces cerevisiae/genetics*; Saccharomyces cerevisiae/metabolism; Time Factors

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