Title: Cutting Edge: Role of NK Cells and Surfactant Protein D in Dendritic Cell Lymph Node Homing: Effects of Ozone Exposure.
Authors: Ge, Moyar Qing; Kokalari, Blerina; Flayer, Cameron H; Killingbeck, Sarah S; Redai, Imre G; MacFarlane 4th, Alexander W; Hwang, Jin W; Kolupoti, Anisha; Kemeny, Michael D; Campbell, Kerry S; Haczku, Angela
Published In J Immunol, (2016 Jan 15)
Abstract: The roles of NK cells, surfactant protein D (SP-D), and IFN-γ, as well as the effect of ozone (O3) inhalation, were studied on recirculation of pulmonary dendritic cells (DC) to the mediastinal lymph nodes. O3 exposure and lack of SP-D reduced NK cell IFN-γ and lung tissue CCL21 mRNA expression and impaired DC homing to the mediastinal lymph nodes. Notably, addition of recombinant SP-D to naive mononuclear cells stimulated IFN-γ release in vitro. Because NKp46, a glycosylated membrane receptor, was necessary for dose-dependent SP-D binding to NK cells in vitro and DC migration in vivo, we speculate that SP-D may constitutively stimulate IFN-γ production by NK cells, possibly via NKp46. This mechanism could then initiate the IFN-γ/IL-12 feedback circuit, a key amplifier of DC lymph node homing. Inhibition of this process during an acute inflammatory response causes DC retention in the peripheral lung tissue and contributes to injury.
PubMed ID: 26673133
MeSH Terms: Animals; Chemotaxis, Leukocyte/drug effects*; Dendritic Cells/immunology*; Flow Cytometry; Interferon-gamma; Killer Cells, Natural/immunology*; Lung/immunology; Lymph Nodes/immunology*; Male; Mice; Mice, Inbred C57BL; Ozone/toxicity*; Pneumonia/immunology; Pulmonary Surfactant-Associated Protein D/immunology*; Real-Time Polymerase Chain Reaction