Title: Visualizing the Path of DNA through Proteins Using DREEM Imaging.

Authors: Wu, Dong; Kaur, Parminder; Li, Zimeng M; Bradford, Kira C; Wang, Hong; Erie, Dorothy A

Published In Mol Cell, (2016 Jan 21)

Abstract: Many cellular functions require the assembly of multiprotein-DNA complexes. A growing area of structural biology aims to characterize these dynamic structures by combining atomic-resolution crystal structures with lower-resolution data from techniques that provide distributions of species, such as small-angle X-ray scattering, electron microscopy, and atomic force microscopy (AFM). A significant limitation in these combinatorial methods is localization of the DNA within the multiprotein complex. Here, we combine AFM with an electrostatic force microscopy (EFM) method to develop an exquisitely sensitive dual-resonance-frequency-enhanced EFM (DREEM) capable of resolving DNA within protein-DNA complexes. Imaging of nucleosomes and DNA mismatch repair complexes demonstrates that DREEM can reveal both the path of the DNA wrapping around histones and the path of DNA as it passes through both single proteins and multiprotein complexes. Finally, DREEM imaging requires only minor modifications of many existing commercial AFMs, making the technique readily available.

PubMed ID: 26774284

MeSH Terms: No MeSH terms associated with this publication