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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Single-Cell Mechanics Provides an Effective Means To Probe in Vivo Interactions between Alveolar Macrophages and Silver Nanoparticles.

Authors: Liu, Ying X; Karsai, Arpad; Anderson, Donald S; Silva, Rona M; Uyeminami, Dale L; Van Winkle, Laura S; Pinkerton, Kent E; Liu, Gang-yu

Published In J Phys Chem B, (2015 Dec 10)

Abstract: Single-cell mechanics, derived from atomic force microscopy-based technology, provides a new and effective means to investigate nanomaterial-cell interactions upon in vivo exposure. Lung macrophages represent initial and important responses upon introducing nanoparticles into the respiratory tract, as well as particle clearance with time. Cellular mechanics has previously proven effective to probe in vitro nanomaterial-cell interactions. This study extends technology further to probe the interactions between primary alveolar macrophages (AM) and silver nanoparticles (AgNPs) upon in vivo exposure. Two types of AgNPs, 20 and 110 nm, were instilled to rat lung at 0.5 mg AgNPs/kg body weight, and allowed 24 h interaction. The consequences of these interactions were investigated by harvesting the primary AMs while maintaining their biological status. Cellular mechanics measurements revealed the diverse responses among AM cells, due to variations in AgNP uptake and oxidative dissolving into Ag(+). Three major responses are evident: zero to low uptake that does not alter cellular mechanics, intracellular accumulation of AgNPs trigger cytoskeleton rearrangement resulting in the stiffening of mechanics, and damage of cytoskeleton that softens the mechanical profile. These effects were confirmed using confocal imaging of F-actin and measurements of reactive oxygen species production. More detailed intracellular interactions will also be discussed on the basis of this study in conjunction with prior knowledge of AgNP toxicity.

PubMed ID: 26562364 Exiting the NIEHS site

MeSH Terms: Animals; Macrophages, Alveolar/cytology; Macrophages, Alveolar/metabolism*; Metal Nanoparticles/chemistry*; Microscopy, Atomic Force; Molecular Probes*; Rats; Reactive Oxygen Species/metabolism; Silver/chemistry*; Single-Cell Analysis*

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