Title: High-Resolution Multi-Photon Imaging of Morphological Structures of Caenorhabditis elegans.
Authors: Bixel, Gabriele M; Fretham, Stephanie J B; Aschner, Michael
Published In Curr Protoc Toxicol, (2015 May 04)
Abstract: In this protocol, we combine two-photon excitation fluorescence with nonlinear optical measurements to reconstruct the three-dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid-body region of Caenorhabditis elegans (C. elegans). Femto-second laser pulses excite second-harmonic generation (SHG) and third-harmonic generation (THG) signals, which show detailed structural information regarding the organization of myofibrils that are arranged around the central pharynx region. The combination of two-photon excitation with SHG and THG imaging is a very powerful tool to study cell morphology, microarchitecture, and tissue arrangement in C. elegans.
PubMed ID: 26344221
MeSH Terms: Animals; Caenorhabditis elegans/anatomy & histology*; Caenorhabditis elegans/ultrastructure; Imaging, Three-Dimensional/methods*; Microscopy, Fluorescence, Multiphoton/methods*; Muscles/anatomy & histology*; Neurons/ultrastructure*; Nonlinear Dynamics; Pharynx/anatomy & histology*