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Title: BMP2 rescues deficient cell migration in Tgfbr3(-/-) epicardial cells and requires Src kinase.

Authors: Allison, Patrick; Espiritu, Daniella; Camenisch, Todd D

Published In Cell Adh Migr, (2016 May 03)

Abstract: During embryogenesis, the epicardium undergoes proliferation, migration, and differentiation into several cardiac cell types which contribute to the coronary vessels. The type III transforming growth factor-β receptor (TGFβR3) is required for epicardial cell invasion and development of coronary vasculature in vivo. Bone Morphogenic Protein-2 (BMP2) is a driver of epicardial cell migration. Utilizing a primary epicardial cell line derived from Tgfbr3(+/+) and Tgfbr3(-/-) mouse embryos, we show that Tgfbr3(-/-) epicardial cells are deficient in BMP2 mRNA expression. Tgfbr3(-/-) epicardial cells are deficient in 2-dimensional migration relative to Tgfbr3(+/+) cells; BMP2 induces cellular migration to Tgfbr3(+/+) levels without affecting proliferation. We further demonstrate that Src kinase activity is required for BMP2 driven Tgfbr3(-/-) migration. BMP2 also requires Src for filamentous actin polymerization in Tgfbr3(-/-) epicardial cells. Taken together, our data identifies a novel pathway in epicardial cell migration required for development of the coronary vessels.

PubMed ID: 26645362 Exiting the NIEHS site

MeSH Terms: Actins/metabolism; Animals; Bone Morphogenetic Protein 2/pharmacology*; Cell Movement/drug effects*; Cell Movement/genetics; Cell Proliferation/drug effects; Gene Expression Profiling; Humans; Mice; Pericardium/cytology*; Polymerization; Proteoglycans/deficiency*; Proteoglycans/metabolism; Receptors, Transforming Growth Factor beta/deficiency*; Receptors, Transforming Growth Factor beta/metabolism; src-Family Kinases/metabolism*

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