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Title: Growth and the Environment of Schizosaccharomyces pombe.

Authors: Petersen, Janni; Russell, Paul

Published In Cold Spring Harb Protoc, (2016 Mar 01)

Abstract: Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures. We discuss the concept of "culture memory," in which the growth state and stress experienced by a strain during storage, propagation, and starter culture preparation can alter experimental outcomes at later stages. We also describe the triggers that are widely used to manipulate signaling through the environment sensing pathways.

PubMed ID: 26933253 Exiting the NIEHS site

MeSH Terms: Culture Media/chemistry*; Microbiological Techniques/methods*; Schizosaccharomyces/growth & development*

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