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Title: Differential epigenetic effects of chlorpyrifos and arsenic in proliferating and differentiating human neural progenitor cells.

Authors: Kim, Hee Yeon; Wegner, Susanna H; Van Ness, Kirk P; Park, Julie Juyoung; Pacheco, Sara E; Workman, Tomomi; Hong, Sungwoo; Griffith, William; Faustman, Elaine M

Published In Reprod Toxicol, (2016 Oct)

Abstract: Understanding the underlying temporal and mechanistic responses to neurotoxicant exposures during sensitive periods of neuronal development are critical for assessing the impact of these exposures on developmental processes. To investigate the importance of timing of neurotoxicant exposure for perturbation of epigenetic regulation, we exposed human neuronal progenitor cells (hNPCs) to chlorpyrifos (CP) and sodium arsenite (As; positive control) during proliferation and differentiation. CP or As treatment effects on hNPCs morphology, cell viability, and changes in protein expression levels of neural differentiation and cell stress markers, and histone H3 modifications were examined. Cell viability, proliferation/differentiation status, and epigenetic results suggest that hNPCs cultures respond to CP and As treatment with different degrees of sensitivity. Histone modifications, as measured by changes in histone H3 phosphorylation, acetylation and methylation, varied for each toxicant and growth condition, suggesting that differentiation status can influence the epigenetic effects of CP and As exposures.

PubMed ID: 27523287 Exiting the NIEHS site

MeSH Terms: Acetylation/drug effects; Arsenic/toxicity; Arsenites/toxicity*; Cell Differentiation/drug effects; Cell Differentiation/genetics; Cell Proliferation/drug effects; Cell Proliferation/genetics; Cells, Cultured; Chlorpyrifos/toxicity*; Cholinesterase Inhibitors/toxicity; Epigenesis, Genetic*; Histones/metabolism; Humans; Insecticides/toxicity; Methylation/drug effects; Neural Stem Cells/drug effects*; Neural Stem Cells/metabolism; Phosphorylation/drug effects; Sodium Compounds/toxicity*

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