Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: A Novel Mechanism of Host-Pathogen Interaction through sRNA in Bacterial Outer Membrane Vesicles.

Authors: Koeppen, Katja; Hampton, Thomas H; Jarek, Michael; Scharfe, Maren; Gerber, Scott A; Mielcarz, Daniel W; Demers, Elora G; Dolben, Emily L; Hammond, John H; Hogan, Deborah A; Stanton, Bruce A

Published In PLoS Pathog, (2016 06)

Abstract: Bacterial outer membrane vesicle (OMV)-mediated delivery of proteins to host cells is an important mechanism of host-pathogen communication. Emerging evidence suggests that OMVs contain differentially packaged short RNAs (sRNAs) with the potential to target host mRNA function and/or stability. In this study, we used RNA-Seq to characterize differentially packaged sRNAs in Pseudomonas aeruginosa OMVs, and to show transfer of OMV sRNAs to human airway cells. We selected one sRNA for further study based on its stable secondary structure and predicted mRNA targets. Our candidate sRNA (sRNA52320), a fragment of a P. aeruginosa methionine tRNA, was abundant in OMVs and reduced LPS-induced as well as OMV-induced IL-8 secretion by cultured primary human airway epithelial cells. We also showed that sRNA52320 attenuated OMV-induced KC cytokine secretion and neutrophil infiltration in mouse lung. Collectively, these findings are consistent with the hypothesis that sRNA52320 in OMVs is a novel mechanism of host-pathogen interaction whereby P. aeruginosa reduces the host immune response.

PubMed ID: 27295279 Exiting the NIEHS site

MeSH Terms: Animals; Bacterial Outer Membrane Proteins/metabolism; Host-Pathogen Interactions/physiology*; Humans; Male; Mice; Mice, Inbred C57BL; Polymerase Chain Reaction; Proteomics; Pseudomonas Infections*; Pseudomonas aeruginosa/pathogenicity; RNA, Small Interfering/metabolism*; RNA, Viral/metabolism*; Respiratory Mucosa/microbiology; Transport Vesicles/genetics; Transport Vesicles/metabolism*

Back
to Top