Title: Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction.
Authors: Zhao, Shuting; Xu, Zhaobin; Wang, Hai; Reese, Benjamin E; Gushchina, Liubov V; Jiang, Meng; Agarwal, Pranay; Xu, Jiangsheng; Zhang, Mingjun; Shen, Rulong; Liu, Zhenguo; Weisleder, Noah; He, Xiaoming
Published In Nat Commun, (2016 10 27)
Abstract: It is difficult to achieve minimally invasive injectable cell delivery while maintaining high cell retention and animal survival for in vivo stem cell therapy of myocardial infarction. Here we show that pluripotent stem cell aggregates pre-differentiated into the early cardiac lineage and encapsulated in a biocompatible and biodegradable micromatrix, are suitable for injectable delivery. This method significantly improves the survival of the injected cells by more than six-fold compared with the conventional practice of injecting single cells, and effectively prevents teratoma formation. Moreover, this method significantly enhances cardiac function and survival of animals after myocardial infarction, as a result of a localized immunosuppression effect of the micromatrix and the in situ cardiac regeneration by the injected cells.
PubMed ID: 27786170
MeSH Terms: Animals; Bioengineering/methods*; Capsules; Cell Aggregation/genetics; Cell Differentiation/genetics; Cells, Cultured; Gene Expression Profiling/methods; Injections; Mice; Mice, Inbred C57BL; Mice, Knockout; Mouse Embryonic Stem Cells/cytology*; Mouse Embryonic Stem Cells/metabolism; Myocardial Infarction/therapy*; Pluripotent Stem Cells/cytology*; Pluripotent Stem Cells/metabolism; Stem Cell Transplantation/methods*