Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

National Institute of Environmental Health Sciences

Use this QR code to view the newest version of this document
Use this QR code to view the newest version of this document

Your Environment. Your Health.

Publication Detail

Title: Quantitative Analysis and Discovery of Lysine and Arginine Modifications.

Authors: Galligan, James J; Kingsley, Philip J; Wauchope, Orrette R; Mitchener, Michelle M; Camarillo, Jeannie M; Wepy, James A; Harris, Peter S; Fritz, Kristofer S; Marnett, Lawrence J

Published In Anal Chem, (2017 01 17)

Abstract: Post-translational modifications (PTMs) affect protein function, localization, and stability, yet very little is known about the ratios of these modifications. Here, we describe a novel method to quantitate and assess the relative stoichiometry of Lys and Arg modifications (QuARKMod) in complex biological settings. We demonstrate the versatility of this platform in monitoring recombinant protein modification of peptide substrates, PTMs of individual histones, and the relative abundance of these PTMs as a function of subcellular location. Lastly, we describe a product ion scanning technique that offers the potential to discover unexpected and possibly novel Lys and Arg modifications. In summary, this approach yields accurate quantitation and discovery of protein PTMs in complex biological systems without the requirement of high mass accuracy instrumentation.

PubMed ID: 27982582 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

Back
to Top