Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Detecting Protein Sulfenylation in Cells Exposed to a Toxicant.

Authors: Wages, Phillip A

Published In Curr Protoc Toxicol, (2017 Feb 01)

Abstract: Protein sulfenylation is a post-translational modification that is linked to many cell signaling networks and specific protein functions, thus the detection of any sulfenylated protein after a toxicological exposure is of importance. Specifically, the detection of protein sulfenylation can provide multiple levels of mechanistic insight towards understanding the impact of a toxicological exposure. For instance, sulfenylation is caused by only a handful of reactive chemical species. Any altered sulfenylation suggests a change in cellular health, and the elucidation of the specific protein target that undergoes sulfenylation can help ascertain downstream targets and associated adverse outcomes. This document describes straightforward approaches to detect protein sulfenylation of total protein as well as individual proteins of interest with a focus on immunoblotting approaches. © 2017 by John Wiley & Sons, Inc.

PubMed ID: 28146279 Exiting the NIEHS site

MeSH Terms: Animals; Cattle; Cell Line; Culture Media; Electrophoresis, Polyacrylamide Gel; Oxidative Stress; Protein Processing, Post-Translational; Proteins/metabolism*; Sulfamerazine/metabolism*

Back
to Top