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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Zebrafish have an ethanol-inducible hepatic 4-nitrophenol hydroxylase that is not CYP2E1-like.

Authors: Hartman, Jessica H; Kozal, Jordan S; Di Giulio, Richard T; Meyer, Joel N

Published In Environ Toxicol Pharmacol, (2017 Sep)

Abstract: Zebrafish are an attractive model organism for toxicology; however, an important consideration in translating between species is xenobiotic metabolism/bioactivation. CYP2E1 metabolizes small hydrophobic molecules, e.g. ethanol, cigarette smoke, and diesel exhaust components. CYP2E1 is thought to only be conserved in mammals, but recent reports identified homologous zebrafish cytochrome P450s. Herein, ex vivo biochemical measurements show that unlike mammals, zebrafish possess a low-affinity 4-nitrophenol hydroxylase (Km ∼0.6 mM) in hepatic microsomes and mitochondria that is inducible only 1.5- to 2-fold by ethanol and is insensitive to 4-methylpyrazole inhibition. In closing, we suggest creating improved models to study CYP2E1 in zebrafish.

PubMed ID: 28728133 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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