Skip Navigation

Publication Detail

Title: The RFamide receptor DMSR-1 regulates stress-induced sleep in C. elegans.

Authors: Iannacone, Michael J; Beets, Isabel; Lopes, Lindsey E; Churgin, Matthew A; Fang-Yen, Christopher; Nelson, Matthew D; Schoofs, Liliane; Raizen, David M

Published In Elife, (2017 01 17)

Abstract: In response to environments that cause cellular stress, animals engage in sleep behavior that facilitates recovery from the stress. In Caenorhabditis elegans, stress-induced sleep(SIS) is regulated by cytokine activation of the ALA neuron, which releases FLP-13 neuropeptides characterized by an amidated arginine-phenylalanine (RFamide) C-terminus motif. By performing an unbiased genetic screen for mutants that impair the somnogenic effects of FLP-13 neuropeptides, we identified the gene dmsr-1, which encodes a G-protein coupled receptor similar to an insect RFamide receptor. DMSR-1 is activated by FLP-13 peptides in cell culture, is required for SIS in vivo, is expressed non-synaptically in several wake-promoting neurons, and likely couples to a Gi/o heterotrimeric G-protein. Our data expand our understanding of how a single neuroendocrine cell coordinates an organism-wide behavioral response, and suggest that similar signaling principles may function in other organisms to regulate sleep during sickness.

PubMed ID: 28094002 Exiting the NIEHS site

MeSH Terms: Animals; Caenorhabditis elegans Proteins/metabolism*; Caenorhabditis elegans/physiology*; Gene Expression Regulation*; Neuropeptides/metabolism*; Receptors, Peptide/metabolism*; Sleep*; Stress, Physiological*

Back
to Top