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Title: Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes That Carry Out DNA Repair.

Authors: LeBlanc, Sharonda; Wilkins, Hunter; Li, Zimeng; Kaur, Parminder; Wang, Hong; Erie, Dorothy A

Published In Methods Enzymol, (2017)

Abstract: Atomic force microscopy (AFM) is a scanning probe technique that allows visualization of single biomolecules and complexes deposited on a surface with nanometer resolution. AFM is a powerful tool for characterizing protein-protein and protein-DNA interactions. It can be used to capture snapshots of protein-DNA solution dynamics, which in turn, enables the characterization of the conformational properties of transient protein-protein and protein-DNA interactions. With AFM, it is possible to determine the stoichiometries and binding affinities of protein-protein and protein-DNA associations, the specificity of proteins binding to specific sites on DNA, and the conformations of the complexes. We describe methods to prepare and deposit samples, including surface treatments for optimal depositions, and how to quantitatively analyze images. We also discuss a new electrostatic force imaging technique called DREEM, which allows the visualization of the path of DNA within proteins in protein-DNA complexes. Collectively, these methods facilitate the development of comprehensive models of DNA repair and provide a broader understanding of all protein-protein and protein-nucleic acid interactions. The structural details gleaned from analysis of AFM images coupled with biochemistry provide vital information toward establishing the structure-function relationships that govern DNA repair processes.

PubMed ID: 28668121 Exiting the NIEHS site

MeSH Terms: DNA Mismatch Repair*; DNA/chemistry; DNA/metabolism*; Equipment Design; Humans; Microscopy, Atomic Force/instrumentation; Microscopy, Atomic Force/methods*; MutL Proteins/chemistry; MutL Proteins/metabolism*; MutS DNA Mismatch-Binding Protein/chemistry; MutS DNA Mismatch-Binding Protein/metabolism*; Protein Binding; Protein Conformation; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/metabolism; Static Electricity; Thermus/chemistry; Thermus/metabolism

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