Title: A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements.

Authors: Orton, Daniel J; Tfaily, Malak M; Moore, Ronald J; LaMarche, Brian L; Zheng, Xueyun; Fillmore, Thomas L; Chu, Rosalie K; Weitz, Karl K; Monroe, Matthew E; Kelly, Ryan T; Smith, Richard D; Baker, Erin S

Published In Anal Chem, (2018 Jan 02)

Abstract: To better understand disease conditions and environmental perturbations, multiomic studies combining proteomic, lipidomic, and metabolomic analyses are vastly increasing in popularity. In a multiomic study, a single sample is typically extracted in multiple ways, and various analyses are performed using different instruments, most often based upon mass spectrometry (MS). Thus, one sample becomes many measurements, making high throughput and reproducible evaluations a necessity. One way to address the numerous samples and varying instrumental conditions is to utilize a flow injection analysis (FIA) system for rapid sample injections. While some FIA systems have been created to address these challenges, many have limitations such as costly consumables, low pressure capabilities, limited pressure monitoring, and fixed flow rates. To address these limitations, we created an automated, customizable FIA system capable of operating at a range of flow rates (∼50 nL/min to 500 μL/min) to accommodate both low- and high-flow MS ionization sources. This system also functions at varying analytical throughputs from 24 to 1200 samples per day to enable different MS analysis approaches. Applications ranging from native protein analyses to molecular library construction were performed using the FIA system, and results showed a highly robust and reproducible platform capable of providing consistent performance over many days without carryover, as long as washing buffers specific to each molecular analysis were utilized.

PubMed ID: 29161511

MeSH Terms: Escherichia coli Proteins/chemistry; Escherichia coli/chemistry; Flow Injection Analysis/instrumentation*; Flow Injection Analysis/methods; Hydrogen-Ion Concentration; Ion Mobility Spectrometry/instrumentation*; Ion Mobility Spectrometry/methods; Mass Spectrometry/instrumentation*; Mass Spectrometry/methods; Soil/chemistry