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National Institute of Environmental Health Sciences

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Publication Detail

Title: Efficient label-free chemiluminescent immunosensor based on dual functional cupric oxide nanorods as peroxidase mimics.

Authors: Li, Juan; Cao, Yue; Hinman, Samuel S; McKeating, Kristy S; Guan, Yiwen; Hu, Xiaoya; Cheng, Quan; Yang, Zhanjun

Published In Biosens Bioelectron, (2018 Feb 15)

Abstract: Dual-functional cupric oxide nanorods (CuONRs) as peroxidase mimics are proposed for the development of a flow-through, label-free chemiluminescent (CL) immunosensor. Forming the basis of this cost-efficient, label-free immunoassay, CuONRs, synthesized using a simple hydrothermal method, were deposited onto epoxy-activated standard glass slides, followed by immobilization of biotinylated capture antibodies through a streptavidin bridge. The CuONRs possess excellent catalytic activity, along with high stability as a solid support. Antigens could then be introduced to the sensing system, forming large immunocomplexes that prevent CL substrate access to the surface, thereby reducing the CL signal in a concentration dependent fashion. Using carcinoembryonic antigen (CEA) as a model analyte, the proposed label-free immunosensor was able to rapidly determine CEA with a wide linear range of 0.1-60ngmL-1 and a low detection limit of 0.05ngmL-1. This nanozyme-based immunosensor is simple, sensitive, cost-efficient, and has the potential to be a very promising platform for fast and efficient biosensing applications.

PubMed ID: 28942213 Exiting the NIEHS site

MeSH Terms: Antibodies, Immobilized/chemistry*; Biomimetic Materials/chemistry*; Biomimetics/economics; Biomimetics/methods; Biosensing Techniques/economics; Biosensing Techniques/methods*; Carcinoembryonic Antigen/blood*; Copper/chemistry*; Humans; Immunoassay/economics; Immunoassay/methods; Limit of Detection; Luminescent Measurements/economics; Luminescent Measurements/methods; Nanotubes/chemistry*; Nanotubes/ultrastructure; Peroxidase/chemistry*

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