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Title: TALEN-Mediated FLAG-Tagging of Endogenous Histone Methyltransferase DOT1L.

Authors: An, Cheng; Zhu, Guangjing; Martos, Suzanne N; Feng, Xue; Zhang, Haimou; Jia, Yankai; Wang, Zhibin

Published In Adv Biosci Biotechnol, (2017 Sep)

Abstract: Histone modification including H3 lysine 79 methylation (H3K79me) plays a key role during gene transcription and DNA damage repair. DOT1L, the sole methyltransferase for three states of H3K79me, is implicated in leukemia, co-lorectal cancer, and dilated cardiomyopathy. However, understanding of DOT1L and H3K79me in these pathways and disease pathogenesis has been limited due to the difficulty of working with DOT1L protein. For instance, locus-specific or genome-wide binding sites of DOT1L revealed by chromatin immunoprecipitation (ChIP)-based methods are necessary for inferring its functions, but high-quality ChIP-grade antibodies are currently not available. Herein we have developed a knock-in approach to tag endogenous DOT1L with 3 × Flag at its C-terminal domain to follow functional analyses. The knock-in was facilitated by using TALENs to induce a targeted double-strand break at the endogenous DOTIL to stimulate local homologous recombination at that site. The single cell colonies with successful knock-in were isolated and verified by different methods. We also demonstrated that tagged DOT1L maintains its normal function in terms of methylation and that the engineered cells would be very useful for further studies.

PubMed ID: 29796335 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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