Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Cellular shear stiffness reflects progression of arsenic-induced transformation during G1.

Authors: Muñoz, Alexandra; Eldridge, Will J; Jakobsen, Nina Munkholt; Sørensen, Helle; Wax, Adam; Costa, Max

Published In Carcinogenesis, (2018 02 09)

Abstract: Cancer cells consistently exhibit decreased stiffness; however, the onset and progression of this change have not been characterized. To study the development of cell stiffness changes, we evaluated the shear stiffness of populations of cells during transformation to a carcinogenic state. Bronchial epithelial cells were exposed to sodium arsenite to initiate early stages of transformation. Exposed cells were cultured in soft agar to further transformation and select for clonal populations exhibiting anchorage-independent growth. Shear stiffness of various cell populations in G1 was assessed using a novel non-invasive assay that applies shear stress with fluid flow and evaluates nanoscale deformation using quantitative phase imaging (QPI). Arsenic-treated cells exhibited reduced stiffness relative to control cells, while arsenic clonal lines, selected by growth in soft agar, were found to have reduced stiffness relative to control clonal lines, which were cultured in soft agar but did not receive arsenic treatment. The relative standard deviation (RSD) of the stiffness of Arsenic clones was reduced compared with control clones, as well as to the arsenic-exposed cell population. Cell stiffness at the population level exhibits potential to be a novel and sensitive framework for identifying the development of cancerous cells.

PubMed ID: 29069374 Exiting the NIEHS site

MeSH Terms: Arsenites/toxicity; Carcinogens/toxicity; Cell Line; Cell Transformation, Neoplastic/chemically induced; Cell Transformation, Neoplastic/pathology*; Epithelial Cells/drug effects; Epithelial Cells/pathology*; G1 Phase; Humans; Respiratory Mucosa/drug effects; Respiratory Mucosa/pathology; Shear Strength/drug effects*; Sodium Compounds/toxicity

Back
to Top