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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: The Cancer/Testes (CT) Antigen HORMAD1 promotes Homologous Recombinational DNA Repair and Radioresistance in Lung adenocarcinoma cells.

Authors: Gao, Yanzhe; Kardos, Jordan; Yang, Yang; Tamir, Tigist Y; Mutter-Rottmayer, Elizabeth; Weissman, Bernard; Major, Michael B; Kim, William Y; Vaziri, Cyrus

Published In Sci Rep, (2018 10 17)

Abstract: The Cancer/Testes (CT) Antigen HORMAD1 is germ cell-restricted and plays developmental roles in generation and processing of meiotic DNA Double Strand Breaks (DSB). Many tumors aberrantly overexpress HORMAD1 yet the potential impact of this CT antigen on cancer biology is unclear. We tested a potential role of HORMAD1 in genome maintenance in lung adenocarcinoma cells. We show that HORMAD1 re-distributes to nuclear foci and co-localizes with the DSB marker γH2AX in response to ionizing radiation (IR) and chemotherapeutic agents. The HORMA domain and C-term disordered oligomerization motif are necessary for localization of HORMAD1 to IR-induced foci (IRIF). HORMAD1-depleted cells are sensitive to IR and camptothecin. In reporter assays, Homologous Recombination (HR)-mediated repair of targeted ISce1-induced DSBs is attenuated in HORMAD1-depleted cells. In Non-Homologous End Joining (NHEJ) reporter assays, HORMAD1-depletion does not affect repair of ISce1-induced DSB. Early DSB signaling events (including ATM phosphorylation and formation of γH2AX, 53BP1 and NBS1 foci) are intact in HORMAD1-depleted cells. However, generation of RPA-ssDNA foci and redistribution of RAD51 to DSB are compromised in HORMAD1-depleted cells, suggesting that HORMAD1 promotes DSB resection. HORMAD1-mediated HR is a neomorphic activity that is independent of its meiotic partners (including HORMAD2 and CCDC36. Bioinformatic analysis of TCGA data show that similar to known HR pathway genes HORMAD1 is overexpressed in lung adenocarcinomas. Overexpression of HR genes is associated with specific mutational profiles (including copy number variation). Taken together, we identify HORMAD1-dependent DSB repair as a new mechanism of radioresistance and a probable determinant of mutability in lung adenocarcinoma.

PubMed ID: 30333500 Exiting the NIEHS site

MeSH Terms: Adenocarcinoma of Lung/drug therapy; Adenocarcinoma of Lung/genetics; Adenocarcinoma of Lung/metabolism*; Adenocarcinoma of Lung/radiotherapy; Antineoplastic Agents/therapeutic use; Camptothecin/therapeutic use; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism*; Cell Line, Tumor; Cisplatin/therapeutic use; DNA End-Joining Repair; Drug Resistance, Neoplasm; Etoposide/therapeutic use; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms/drug therapy; Lung Neoplasms/genetics; Lung Neoplasms/metabolism*; Lung Neoplasms/radiotherapy; Radiation, Ionizing*; Recombinational DNA Repair*

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