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Title: Paper-based Transwell assays: an inexpensive alternative to study cellular invasion.

Authors: Kenney, Rachael M; Loeser, Adam; Whitman, Nathan A; Lockett, Matthew R

Published In Analyst, (2018 Dec 17)

Abstract: Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.

PubMed ID: 30328422 Exiting the NIEHS site

MeSH Terms: Animals; Biological Assay/instrumentation*; Biological Assay/methods; Cattle; Cell Line, Tumor; Cell Movement*; Humans; Neoplasm Invasiveness*; Paper*; Reproducibility of Results

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