Title: Supernumerary Incisors in CB6F1 Mice Conditioned with Chemotherapy and Total Body Irradiation before Bone Marrow Transplantation.

Authors: Doane, Cynthia J; Patil, Karuna; Hoffman, Emely A; Stokes, Jessica; Katsanis, Emmanuel; Besselsen, David G

Published In Comp Med, (2018 Oct 01)

Abstract: Multiple adult female CB6F1 mice presented with supernumerary incisors after preconditioning with chemotherapy and total body irradiation for bone marrow transplantation (BMT). Mice received nonmyeloablative total body irradiation (3 Gy) and either cyclophosphamide or bendamustine, followed by BMT and posttransplantation cyclophosphamide or bendamustine. Here we describe the clinical presentation, μCT findings, and histopathologic evaluation of the affected mice. These analyses confirmed the gross diagnosis and revealed details of the abnormal tooth morphology. We surmise that the combination of total body irradiation and chemotherapy resulted in the abnormal formation of supernumerary incisors. Supernumerary teeth should be considered as a potential confounding factor in tracking weight loss after BMT. These conditions can be managed to allow animals to reach their intended scientific endpoint.

PubMed ID: 30208992

MeSH Terms: Animals; Bendamustine Hydrochloride/adverse effects; Bendamustine Hydrochloride/therapeutic use; Bone Marrow Transplantation/veterinary; Cyclophosphamide/adverse effects; Cyclophosphamide/therapeutic use; Female; Immunosuppressive Agents/adverse effects*; Immunosuppressive Agents/therapeutic use; Incisor/diagnostic imaging*; Mice; Rodent Diseases/diagnostic imaging; Rodent Diseases/etiology*; Tooth, Supernumerary/etiology; Tooth, Supernumerary/veterinary*; Whole-Body Irradiation/adverse effects*