Title: Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms.
Authors: Fritsch, Clark; Gout, Jean-Francois Pierre; Vermulst, Marc
Published In J Vis Exp, (2018 09 13)
Abstract: Accurate transcription is required for the faithful expression of genetic information. Surprisingly though, little is known about the mechanisms that control the fidelity of transcription. To fill this gap in scientific knowledge, we recently optimized the circle-sequencing assay to detect transcription errors throughout the transcriptome of Saccharomyces cerevisiae, Drosophila melanogaster, and Caenorhabditis elegans. This protocol will provide researchers with a powerful new tool to map the landscape of transcription errors in eukaryotic cells so that the mechanisms that control the fidelity of transcription can be elucidated in unprecedented detail.
PubMed ID: 30272673
MeSH Terms: Animals; Eukaryotic Cells/metabolism*; Genomics/methods*; Transcriptome