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Publication Detail

Title: Mitochondrial free calcium transients during excitation-contraction coupling in rabbit cardiac myocytes.

Authors: Chacon, E; Ohata, H; Harper, I S; Trollinger, D R; Herman, B; Lemasters, J J

Published In FEBS Lett, (1996 Mar 11)

Abstract: Mitochondrial free Ca2+ may regulate mitochondrial ATP production during cardiac exercise. Here, using laser scanning confocal microscopy of adult rabbit cardiac myocytes co-loaded with Fluo-3 to measure free Ca2+ and tetramethylrhodamine methylester to identify mitochondria, we measured cytosolic and mitochondrial Ca2+ transients during the contractile cycle. In resting cells, cytosolic and mitochondrial Fluo-3 signals were similar. During electrical pacing, transients of Fluo-3 fluorescence occurred in both the cytosolic and mitochondrial compartments. Both the mitochondrial and the cytosolic transients were potentiated by isoproterenol. These experiments show directly that mitochondrial free Ca2+ rises and falls during excitation-contraction coupling in cardiac myocytes and that changes of mitochondrial Ca2+ are kinetically competent to regulate mitochondrial metabolism on a beat-to-beat basis.

PubMed ID: 8612759 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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