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National Institute of Environmental Health Sciences

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Publication Detail

Title: Biochemical Studies and Molecular Dynamic Simulations Reveal the Molecular Basis of Conformational Changes in DNA Methyltransferase-1.

Authors: Ye, Fei; Kong, Xiangqian; Zhang, Hao; Liu, Yan; Shao, Zhiyuan; Jin, Jia; Cai, Yi; Zhang, Rukang; Li, Linjuan; Zhang, Yang W; Liu, Yu-Chih; Zhang, Chenhua; Xie, Wenbing; Yu, Kunqian; Ding, Hong; Zhao, Kehao; Chen, Shijie; Jiang, Hualiang; Baylin, Stephen B; Luo, Cheng

Published In ACS Chem Biol, (2018 03 16)

Abstract: DNA methyltransferase-1 (DNMT1) plays a crucial role in the maintenance of genomic methylation patterns. The crystal structure of DNMT1 was determined in two different states in which the helix that follows the catalytic loop was either kinked (designated helix-kinked) or well folded (designated helix-straight state). Here, we show that the proper structural transition between these two states is required for DNMT1 activity. The mutations of N1248A and R1279D, which did not affect interactions between DNMT1 and substrates or cofactors, allosterically reduced enzymatic activities in vitro by decreasing kcat/ Km for AdoMet. The crystallographic data combined with molecular dynamic (MD) simulations indicated that the N1248A and R1279D mutants bias the catalytic helix to either the kinked or straight conformation. In addition, genetic complementation assays for the two mutants suggested that disturbing the conformational transition reduced DNMT1 activity in cells, which could act additively with existing DNMT inhibitors to decrease DNA methylation. Collectively, our studies provide molecular insights into conformational changes of the catalytic helix, which is essential for DNMT1 catalytic activity, and thus aid in better understanding the relationship between DNMT1 dynamic switching and enzymatic activity.

PubMed ID: 29381856 Exiting the NIEHS site

MeSH Terms: Animals; Catalytic Domain; Crystallography, X-Ray; DNA (Cytosine-5-)-Methyltransferase 1/chemistry*; DNA (Cytosine-5-)-Methyltransferase 1/genetics; DNA Methylation/genetics; Humans; Molecular Dynamics Simulation*; Mutation; Protein Conformation

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