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National Institute of Environmental Health Sciences

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Publication Detail

Title: A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing.

Authors: Li, Jiannan; Tan, Wen; Xiao, Wenwu; Carney, Randy P; Men, Yongfan; Li, Yuanpei; Quon, Gerald; Ajena, Yousif; Lam, Kit S; Pan, Tingrui

Published In Anal Chem, (2018 12 04)

Abstract: Traditional high-throughput drug combination screening requires automatic pipetting of drugs into high-density microtiter plates. Here, a drug-on-pillar platform is proposed for efficient combination drug screening. Using the proposed approach, combination drug screening can be carried out in a plug-and-play manner, allowing for high-throughput screening of large permutations of drug combinations at various concentrations, such that drug dispensing and cell-based screening can be temporally separated and therefore can potentially be performed at distant laboratories. The dispensing is implemented using our recently developed microfluidic pneumatic printing platform, which features a low-cost disposable cartridge that minimizes cross contamination. Moreover, our previously developed drug nanoformulation method with amphiphilic telodendrimers has been utilized to maintain drug stability in a dry form, allowing for convenient drug storage, shipping, and subsequent rehydration. Combining the features described above, we have implemented a 1260-spot drug combination array to study the effect of paired drugs against MDA-MB-231 triple negative human breast cancer cells. This study supports the feasibility of the drug-on-pillar platform for combination drug screening and has provided valuable insight into drug combination efficacy against breast cancer.

PubMed ID: 30358386 Exiting the NIEHS site

MeSH Terms: Antineoplastic Agents/chemistry; Antineoplastic Agents/pharmacology*; Cell Proliferation/drug effects; Cell Survival/drug effects; Dose-Response Relationship, Drug; Doxorubicin/chemistry; Doxorubicin/pharmacology*; Drug Combinations; Drug Evaluation, Preclinical; Drug Screening Assays, Antitumor; Humans; Microfluidic Analytical Techniques*; Printing, Three-Dimensional*; Structure-Activity Relationship; Triple Negative Breast Neoplasms/drug therapy*; Triple Negative Breast Neoplasms/pathology; Tumor Cells, Cultured

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