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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Development of an immunoassay for the detection of carbaryl in cereals based on a camelid variable heavy-chain antibody domain.

Authors: Liu, Zhiping; Wang, Kai; Wu, Sha; Wang, Zhanhui; Ding, Guochun; Hao, Xiujing; Li, Qing X; Li, Ji; Gee, Shirley J; Hammock, Bruce D; Xu, Ting

Published In J Sci Food Agric, (2019 Jul)

Abstract: BACKGROUND: The variable domain of camelid heavy-chain antibodies (VHH) is increasingly being adapted to detect small molecules in various matrices. The insecticide carbaryl is widely used in agriculture while its residues have posed a threat to food safety and human health. RESULTS: VHHs specific for carbaryl were generated from an alpaca immunized with the hapten CBR1 coupled to keyhole limpet hemocyanin. An enzyme-linked immunosorbent assay (ELISA) based on the VHH C1 and the coating antigen CBR2-BSA was developed for the detection of carbaryl in cereals. This assay, using an optimized assay buffer (pH 6.5) containing 10% methanol and 0.8% NaCl, has a half-maximum signal inhibition concentration of 5.4 ng mL-1 and a limit of detection (LOD) of 0.3 ng mL-1 for carbaryl, and shows low cross reactivity (≤0.8%) with other tested carbamates. The LOD of carbaryl using the VHH-based ELISA was 36 ng g-1 in rice and maize and 72 ng g-1 in wheat. Recoveries of carbaryl in spiked rice, maize and wheat samples were in the range of 81-106%, 96-106% and 83-113%, respectively. Relative standard deviations of repeatability and intra-laboratory reproducibility were in the range of 0.8-9.2% and 2.9-9.7%, respectively. CONCLUSION: The VHH-based ELISA was highly effective in detecting carbaryl in cereal samples after simple sample extraction and dilution. © 2019 Society of Chemical Industry.

PubMed ID: 30851058 Exiting the NIEHS site

MeSH Terms: Animals; Camelids, New World; Carbaryl/analysis*; Edible Grain/chemistry; Enzyme-Linked Immunosorbent Assay/methods*; Food Contamination/analysis; Immunoglobulin Heavy Chains/analysis; Immunoglobulin Heavy Chains/immunology; Insecticides/analysis*; Limit of Detection; Single-Chain Antibodies/analysis; Single-Chain Antibodies/immunology; Triticum/chemistry*; Zea mays/chemistry*

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