Skip Navigation

Publication Detail

Title: Targeted Quantitative Kinome Analysis Identifies PRPS2 as a Promoter for Colorectal Cancer Metastasis.

Authors: Miao, Weili; Wang, Yinsheng

Published In J Proteome Res, (2019 05 03)

Abstract: Kinases are among the most important families of enzymes involved in cell signaling. In this study, we employed a recently developed parallel-reaction monitoring (PRM)-based targeted proteomic method to examine the reprogramming of the human kinome during colorectal cancer (CRC) metastasis. We were able to quantify the relative expression of 299 kinase proteins in a pair of matched primary/metastatic CRC cell lines. We also found that, among the differentially expressed kinases, phosphoribosyl pyrophosphate synthetase 2 (PRPS2) promotes the migration and invasion of cultured CRC cells through regulating the activity of matrix metalloproteinase 9 (MMP-9) and the expression of E-cadherin. Moreover, we found that the up-regulation of PRPS2 in metastatic CRC cells could be induced by the MYC proto-oncogene. Together, our unbiased kinome profiling approach led to the identification, for the first time, of PRPS2 as a promoter for CRC metastasis.

PubMed ID: 30908912 Exiting the NIEHS site

MeSH Terms: Antigens, CD/genetics*; Antigens, CD/metabolism; Cadherins/genetics*; Cadherins/metabolism; Cell Line, Tumor; Cell Movement/genetics; Colorectal Neoplasms/genetics*; Colorectal Neoplasms/metabolism; Colorectal Neoplasms/pathology; Gene Expression Profiling; Gene Expression Regulation, Neoplastic*; Humans; Lymphatic Metastasis; Matrix Metalloproteinase 9/genetics*; Matrix Metalloproteinase 9/metabolism; Neoplasm Invasiveness; Protein Kinases/classification; Protein Kinases/genetics*; Protein Kinases/metabolism; Proto-Oncogene Proteins c-myc/genetics; Proto-Oncogene Proteins c-myc/metabolism; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; Ribose-Phosphate Pyrophosphokinase/antagonists & inhibitors; Ribose-Phosphate Pyrophosphokinase/genetics*; Ribose-Phosphate Pyrophosphokinase/metabolism; Tumor Cells, Cultured

Back
to Top