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National Institute of Environmental Health Sciences

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Publication Detail

Title: Tissue-specific quantification and localization of androgen and estrogen receptors in prostate cancer.

Authors: Sehgal, Priyanka D; Bauman, Tyler M; Nicholson, Tristan M; Vellky, Jordan E; Ricke, Emily A; Tang, Weiping; Xu, Wei; Huang, Wei; Ricke, William A

Published In Hum Pathol, (2019 07)

Abstract: Androgens and estrogens, working together, promote prostate cancer (PRCA) initiation and progression, with androgens acting via androgen receptor (AR) and estrogens acting primarily through estrogen receptor α (ERα). While the interplay between these steroid hormones has been established, the interaction between steroid hormone receptors in prostatic disease remains unstudied. The goal of this study was to objectively determine the incidence, stage specificity, and tissue/cell type specificity of AR and ERα expression, both independently and simultaneously, during the progression of PRCA. Using multiplexed immunohistochemistry and multispectral imaging analysis, AR, ERα, and smooth muscle α-actin expression was detected and quantitated in benign prostate tissue (BPT), high-grade prostatic intraepithelial neoplasia (HGPIN), PRCA, and metastasis (MET) from patient specimens (n=340). Epithelial AR expression was significantly increased in HGPIN, PRCA, and MET compared with BPT, whereas ERα expression in epithelial and stromal cells was highest in HGPIN. With analysis of AR and ERα coexpression, we identified a unique population of double-positive (AR+/ERα+) cells that increased in HGPIN specimens in both the stroma and the epithelium. Double-negative (AR-/ERα-) cells significantly decreased across PRCA progression, from 65% in BPT to 30% in MET. Preliminary analysis of this AR+/ERα+ population indicates potential cell type specificity in smooth muscle α-actin-negative stromal cells. This study demonstrates stage-, tissue-, and cell type-specific AR and ERα expression changes during PRCA progression, both independently and coexpressed. A more complete understanding of steroid hormones and their receptors in the initiation and progression of prostatic disease may elucidate improved strategies for PRCA prevention or therapy.

PubMed ID: 31054895 Exiting the NIEHS site

MeSH Terms: Biomarkers, Tumor/analysis*; Disease Progression; Humans; Male; Prostatic Neoplasms/metabolism*; Prostatic Neoplasms/pathology*; Receptors, Androgen/analysis; Receptors, Androgen/biosynthesis*; Receptors, Estrogen/analysis; Receptors, Estrogen/biosynthesis*

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