Title: Establishment and characterization of a multi-purpose large animal exposure chamber for investigating health effects.
Authors: Peng, Xinze; Maltz, Mia R; Botthoff, Jon K; Aronson, Emma L; Nordgren, Tara M; Lo, David D; Cocker, David R
Published In Rev Sci Instrum, (2019 Mar)
Abstract: Air pollution poses a significant threat to the environment and human health. Most in vivo health studies conducted regarding air pollutants, including particulate matter (PM) and gas phase pollutants, have been either through traditional medical intranasal treatment or using a tiny chamber, which limit animal activities. In this study, we designed and tested a large, whole-body, multiple animal exposure chamber with uniform dispersion and exposure stability for animal studies. The chamber simultaneously controls particle size distribution and PM mass concentration. Two different methods were used to generate aerosol suspension through either soluble material (Alternaria extract), liquid particle suspension (nanosilica solution), or dry powder (silica powder). We demonstrate that the chamber system provides well controlled and characterized whole animal exposures, where dosage is by inhalation of particulate matter.
PubMed ID: 30927824
MeSH Terms: Air Pollution/analysis*; Animals; Environmental Monitoring/instrumentation*; Equipment Design; Industrial Waste/analysis; Nanoparticles/chemistry; Particle Size; Silicon Dioxide/analysis; Silicon Dioxide/chemistry; Swine