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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Publication Detail

Title: Soy Isoflavone Supplementation Increases Long Interspersed Nucleotide Element-1 (LINE-1) Methylation in Head and Neck Squamous Cell Carcinoma.

Authors: Rozek, Laura S; Virani, Shama; Bellile, Emily L; Taylor, Jeremy M G; Sartor, Maureen A; Zarins, Katie R; Virani, A; Cote, C; Worden, Francis P; Mark, Mark E Prince; McLean, Scott A; Duffy, Sonya A; Yoo, George H; Saba, Nabil F; Shin, Dong M; Kucuk, Omer; Wolf, Gregory T

Published In Nutr Cancer, (2019)

Abstract: Soy isoflavones have been suggested as epigenetic modulating agents with effects that could be important in carcinogenesis. Hypomethylation of LINE-1 has been associated with head and neck squamous cell carcinoma (HNSCC) development from oral premalignant lesions and with poor prognosis. To determine if neoadjuvant soy isoflavone supplementation could modulate LINE-1 methylation in HNSCC, we undertook a clinical trial.Thirty-nine patients received 2-3 weeks of soy isoflavone supplements (300 mg/day) orally prior to surgery. Methylation of LINE-1, and 6 other genes was measured by pyrosequencing in biopsy, resection, and whole blood (WB) specimens. Changes in methylation were tested using paired t tests and ANOVA. Median follow up was 45 months.LINE-1 methylation increased significantly after soy isoflavone (P < 0.005). Amount of change correlated positively with days of isoflavone taken (P = 0.04). Similar changes were not seen in corresponding WB samples. No significant changes in tumor or blood methylation levels were seen in the other candidate genes.This is the first demonstration of in vivo increases in tissue-specific global methylation associated with soy isoflavone intake in patients with HNSCC. Prior associations of LINE-1 hypomethylation with genetic instability, carcinogenesis, and prognosis suggest that soy isoflavones maybe potential chemopreventive agents in HNSCC.

PubMed ID: 30862188 Exiting the NIEHS site

MeSH Terms: DNA Methylation/drug effects*; Dietary Supplements*; Female; Head and Neck Neoplasms/drug therapy*; Humans; Isoflavones/pharmacology*; Long Interspersed Nucleotide Elements/drug effects*; Male; Middle Aged; Soybeans; Squamous Cell Carcinoma of Head and Neck/drug therapy*

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