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Title: Rapid detection of urokinase plasminogen activator using flexible paper-based graphene-gold platform.

Authors: Sharma, Bipin; Parajuli, Prakash; Podila, Ramakrishna

Published In Biointerphases, (2020 02 04)

Abstract: Many studies have shown that urokinase plasminogen activator (uPA) is causally involved in promoting cancer invasion and metastasis. Thus, monitoring uPA levels could be very useful in cancer diagnosis, identification of initial metastasis, and guiding cancer treatment. Here, the authors developed a novel and scalable uPA sensor based on a graphene-gold nanoparticle platform that uses fluorescence of quantum dots to rapidly (<1 h) detect uPA up to 100 pM. Indeed, the authors' sensor is highly selective and showed an ability to sense up to 100 pM uPA even in the presence of complex biological milieu such as the fetal bovine serum.

PubMed ID: 32019314 Exiting the NIEHS site

MeSH Terms: Antibodies/chemistry; Antibodies/immunology; Biosensing Techniques/methods; Density Functional Theory; Gold/chemistry*; Graphite/chemistry*; Humans; Immunoassay; Limit of Detection; Metal Nanoparticles/chemistry*; Neoplasms/diagnosis; Paper*; Recombinant Proteins/analysis; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Urokinase-Type Plasminogen Activator/analysis*; Urokinase-Type Plasminogen Activator/immunology

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