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Title: Characterizing the activity of abundant, diverse and active CRISPR-Cas systems in lactobacilli.

Authors: Crawley, Alexandra B; Henriksen, Emily D; Stout, Emily; Brandt, Katelyn; Barrangou, Rodolphe

Published In Sci Rep, (2018 Aug 01)

Abstract: CRISPR-Cas systems provide immunity against phages and plasmids in bacteria and archaea. Despite the popularity of CRISPR-Cas9 based genome editing, few endogenous systems have been characterized to date. Here, we sampled 1,262 publically available lactobacilli genomes found them to be enriched with CRISPR-Cas adaptive immunity. While CRISPR-Cas is ubiquitous in some Lactobacillus species, CRISPR-Cas content varies at the strain level in most Lactobacillus species. We identified that Type II is the most abundant type across the genus, with II-A being the most dominant sub-type. We found that many Type II-A systems are actively transcribed, and encode spacers that efficiently provide resistance against plasmid uptake. Analysis of various CRISPR transcripts revealed that guide sequences are highly diverse in terms of crRNA and tracrRNA length and structure. Interference assays revealed highly diverse target PAM sequences. Lastly, we show that these systems can be readily repurposed for self-targeting by expressing an engineered single guide RNA. Our results reveal that Type II-A systems in lactobacilli are naturally active in their native host in terms of expression and efficiently targeting invasive and genomic DNA. Together, these systems increase the possible Cas9 targeting space and provide multiplexing potential in native hosts and heterologous genome editing purpose.

PubMed ID: 30068963 Exiting the NIEHS site

MeSH Terms: Bacteriophages/genetics; CRISPR-Cas Systems*; Gene Expression Profiling; Genetic Variation; Genome, Bacterial; Lactobacillus/enzymology*; Lactobacillus/genetics*; Plasmids; RNA, Guide, CRISPR-Cas Systems/genetics; Recombination, Genetic*

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