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Title: Quantitative Detection of Fipronil and Fipronil-Sulfone in Sera of Black-Tailed Prairie Dogs and Rats after Oral Exposure to Fipronil by Camel Single-Domain Antibody-Based Immunoassays.

Authors: Wang, Kai; Vasylieva, Natalia; Wan, Debin; Eads, David A; Yang, Jun; Tretten, Tyler; Barnych, Bogdan; Li, Ji; Li, Qing X; Gee, Shirley J; Hammock, Bruce D; Xu, Ting

Published In Anal Chem, (2019 Jan 15)

Abstract: The insecticide fipronil can be metabolized to its sulfone in mammalian species. Two camel single-domain antibodies (VHHs) F1 and F6, selective to fipronil and fipronil-sulfone, respectively, were generated and used to develop enzyme linked immunosorbent assays (ELISAs) for the detection of the two compounds in the sera of black-tailed prairie dogs and rats. The limits of detection of fipronil and fipronil-sulfone in the rodent sera by the corresponding ELISAs were 10 and 30 ng mL-1, and the linear ranges were 30-1000 and 75-2200 ng mL-1. ELISAs showed a good recovery for fipronil and fipronil-sulfone cospiked in the control sera of the black-tailed prairie dogs (90-109%) and rats (93-106%). The VHH-based ELISAs detected fipronil and fipronil-sulfone in the sera of the rodents that received a repeated oral administration of fipronil. The average concentration of fipronil-sulfone was approximately 3.2-fold higher than fipronil in the prairie dog sera (1.15 vs 0.36 μg mL-1) and rat sera (1.77 vs 0.53 μg mL-1). ELISAs agreed well with a liquid chromatography-mass spectrometry method for the quantification of both fipronil and fipronil-sulfone in real serum samples. Fipronil-sulfone was identified as the predominant metabolite of fipronil in the black-tailed prairie dog and rat sera.

PubMed ID: 30521755 Exiting the NIEHS site

MeSH Terms: Administration, Oral; Animals; Enzyme-Linked Immunosorbent Assay/methods*; Immunization; Insecticides/administration & dosage; Insecticides/blood*; Insecticides/immunology; Insecticides/metabolism; Pyrazoles/administration & dosage; Pyrazoles/blood*; Pyrazoles/immunology; Pyrazoles/metabolism; Rats; Sciuridae; Single-Domain Antibodies/immunology*

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