Title: Ultrahigh-Density Screens for Genome-Wide Yeast EMAPs in a Single Plate.
Authors: Licon, Katherine; Shen, John Paul; Munson, Brenton P; Michaca, Manuel; Fassino, Cole; Fassino, Luke; Kreisberg, Jason F; Ideker, Trey
Published In Methods Mol Biol, (2019)
Abstract: Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.
PubMed ID: 31602605
MeSH Terms: Epistasis, Genetic/genetics*; Genome, Fungal/genetics*; Saccharomyces cerevisiae/genetics*